Archive for September, 2007

Metabolism of Carbohydrates, Lipids and Proteins

Cells take on food in the form of carbohydrates, lipids and proteins and converts the energy it contains into energy carried in the molecule ATP.

Food molecules are modified by a large number of reactions which occur in small steps, each catalysed by an enzyme.
These are the basis of metabolism. Notice that all three types of food molecules (carbohydrates, lipids and proteins) pass through a common pathway to make ATP called the citric acid cycle (tricarboxylic acid, Krebs cycle) and electron transport chain.
These 2 processes occur in the mitochondria.

Process of making ATP in the mitochondria is called oxidative phosphorylation.
Making ATP outside the mitochondria are relatively small without the presence of air and occurs via glycolysis and fermentation which is also called substrate-level phosphorylation.
(Oxygen is needed to make ATP in the respiratory chain and carbon from the food is released in the form of carbon dioxide. This is why we breath oxygen and breathe out carbon dioxide)

Metabolism also allows the inter-conversion of different types of food molecules. For example if proteins are low in a diet they can be made from carbohydrates or lipids and vice versa. Interconversion between carbohydrates, lipids and proteins occur via the key molecules pyruvic acid and acetyl CoA.

Citric Acid Cycle

The citric acid cycle is a cyclic series of enzyme catalysed reactions that are of fundamental important to metabolism of aerobic organisms. Enzymes for the citric acid cycle are found in the mitochondrion.

2 carbon acetyl-CoA, react with four carbon oxaloacetate to form 6 carbon citrate. In a series of seven reactions, this is reconverted to oxaloacetate and produces 2 molecules of carbon dioxide. But more importantly, the cycle produces 3 molecules of NADH and 1 mole of FADH2, which are subsequently used in electron transport chain to generate ATP.

Food Texture

Food Texture

It is defined in many ways, in which can be described by various structural elements of food are arranged into a micro and/or macrostructure, and external visual display of this results from these structures.

Most of foods are complex mixtures of materials of diverse physical and chemical properties. Because of this, the foods cover a wide range of physical properties from fluid Newtonian materials to the most complex disperse systems with semi-solid character.

How can Food Texture be evaluated
(Mechanical tests or sensory analysis)

  • Light and electron microscopy
  • Xray diffraction analysis, gives a picture with reference to crystalline structure
  • Differential scanning calorimetry, which provides information about melting and solidifcation and other phase transitions
  • Particle size analysis and sedimentation methods provide information about particle size distribution and particle shape.

Due to the complexity of foods texture of food cannot always be described easily and is difficult to establish a uniform measure for texture.

Terms used in textural properties

  • Consistancy with aspects of texture that relats to flow and deformation
  • Hardness can be defined as resistance of deformation
  • Firmness, could be the same as hardness but can be used to describe the property of a substance able to resistance deformation under its own weight
  • Brittleness, property of fracturing.